Part:BBa_K1150043
uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3
| pH1:tracrRNA pU6:DR_dummy_DR | |
|---|---|
| Function | two small RNAs giving rise to
specific binding of dCas9 to two VEGF loci |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Organism | Streptococcus pyogenes |
| Source | Feng Zhang, Addgene |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Usage and Biology
By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the [http://2013.igem.org/Team:Freiburg/Project/effector dCas9 derivates] at once. The basis of the device is the RNAimer.
Here, we combined BBa_K1150037 and BBa_K1150038.
Sequence
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
Illegal BglII site found at 1061 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216
Illegal BsaI.rc site found at 1053
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.
| None |